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Autoimmune Diagnostics

Direct and Indirect ELISA

In principle, there are two possible approaches to the implementation of an ELISA test: for direct ELISA, antibodies directed against the protein being sought are bound to the plate. The protein being detected binds to these antibodies and can be detected in a second step by a second enzyme-marked antibody.

The detection of (auto)antibodies is based on indirect ELISA. For this type of test, the microtitre plate is first coated with the autoantigen to which these antibodies bind.

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Fig.1
Comparison of indirect and direct ELISA
© FIZ CHEMIE

The following video demonstrates the steps for carrying out an ELISA test in the laboratory

Fig.2
ELISA test in the laboratory
© Orgentec Diagnostika
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